![]() ![]() Data management (storage/backup, meta-table management, GEO/dbGap submission), You can use it for Single Cell CNV, Single Cell Gene Expression, Single Cell Immune Profiling, and Single Cell ATAC.Īt this stage, the program will primarily provide NGS data analysis service, including but not limited to: We also provide a single-cell genomics platform: 10x Genomics CHROMIUM CONTROLLER. It’s an affordable, secured, and customized service that both you and your data deserve. For later, we will take care of your data with seamless service, from data management, data backup, data QC, downstream NGS analysis, till the final uploading to GEO/dbGap before your publication. You can choose either to upload the data to Illumina’s cloud storage or save it to the designated space on Partners’ ERISone high-performance computing cluster. Turn around time is provided during the discussion and development phase.As part of the service that our NeuroTech Studio provides, our customers can access the brand new Illumina NextSeq 550 sequencing machine. On the day of processing, you must be available for a phone call, email, or Zoom meeting to discuss results. REQUIRED: Enclose tube(s) inside a small biohazard bag. ![]() Recommendation: Coordinate within your lab to reduce multiple deliveries to a single drop-off reservation whenever possible.ĭeposit the sample inside the cooler outside of the facility door on your reserved day and time. REQUIRED: If you can not make your reservation, cancel it as soon as possible and select a new time slot. Within the reservation, include the email of other lab members who should be consulted or notified with results. Within the service request, including the email of other lab members who should be consulted or notified with results. Schedule a reservation 24 hours in advance by selecting a day and time from the Sample Processing Calendar and attach your completed Excel Submission Form. Go to InsideND, search for iLab Core Facility Management, and sign in using University of Notre Dame credentials. iLab sends us a notification about scheduled reservations. ILab Core Facility Management: Reserve a sample drop-off day/time. Sample requirements are specific to the project and will be outlined during the discussion and development phase prior to confirming and scheduling. Sample Information and QC: Download and complete all fields to submit your samples for evaluation. Project Information: Download and complete all fields to request guide discussion and development of your request. Single-Cell Library Preparation SubmissionsĬore Facility will perform its own evaluation of the samples to confirm (1) concentration and (2) purity using an automated cell counter. Once your request is confirmed and scheduled, follow the instructions for library preparation below. Before initiating your experiment, contact Melissa Stephens to discuss project specifics, receive a quote, and coordinate scheduling. Do not leave samples if the cooler is absent we are not responsible for damage or loss.Ĭonsultation is required for this service. On designated days and reserved times, there will be a cooler outside the facility door to receive samples. Access to facility space is restricted to facility staff. GBCF iLab video tutorials and user guide available on Resource tab.Ĭommunicate with facility staff by email, phone, or Zoom appointment. ![]() Go to InsideND and search for iLab Core Facility Management. ILab transition complete as of January 06, 2021. Internal Users: Access Galvin Life Science Center via the main entrance during regular business days and hours. We are accepting a limited number of requests until further notice. New Policies and ProceduresĮxternal Users: Contact Melissa Stephens. View internal and external service rates. Long Range (Linked-Read) Genome sequencing.Service OptionsĪ variety of library preparation options are available for a wide range of study goals. Researchers are encouraged to contact the GBCF with requests for other services that are not listed below: This enables the discovery of gene expression dynamics and molecular profiling of individual cell types, elucidation of gene regulatory mechanisms, study of disease pathogens, and characterization of genomic structure. The 10X Chromium Controller is a massive partitioning and barcoding system with a reagent delivery method that facilitates thousands of micro-reactions in parallel. The ability to examine genomic and transcriptomic patterns at the level of single cells is rapidly becoming an essential tool for cell biology, cancer, immunology, and neuroscience research. ![]()
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